AIBNi005-A

Reviewed by Australian Stem Cell Registry

Alternative Names: GENIE 70

Details

Description

This induced pluripotent stem cell line was reprogrammed from the peripheral blood mononuclear cells of a female epilepsy patient using non-integrating sendai viral vectors. AIBNi005-A is described as having a normal karyotype. Expression of OCT4, SOX2, NANOG, TRA-1-60 and TRA-1-81 in undifferentiated cells, and directed differentiation to endoderm, mesoderm and ectoderm have been reported.

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
epilepsy

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2021 Generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells of three drug resistant and three drug responsive epilepsy patients

Line Custodianship

Cell Line Maintainer

Ernst Wolvetang

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia
View all cell lines from this group

Cell Line Producer

Australian Institute for Bioengineering and Nanotechnology

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia

Source

Donor Derived

Age: 30-34

Biological Sex: Female

Disease: MONDO:0005027
Label: epilepsy
Definition: A brain disorder characterized by episodes of abnormally increased neuronal discharge resulting in transient episodes of sensory or motor neurological dysfunction, or psychic dysfunction. These episodes may or may not be associated with loss of consciousness or convulsions.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2020

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Ethics

Ethics Number: 2019002273

Institution Human Research Ethics Council: The University of Queensland Human Ethics Research Office

Approval Date: Jan. 1, 2019

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XX

Karyotype Method: G-banding

Summary: Karyotyping occurred from passage 9 onwards at a resolution of 400bphs. Fifteen metaphase spreads were analysed.

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
HIV 1 Negative
HIV 2 Negative
HEP B Negative
HEP C Negative
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
POU5F1 (OCT-4) Immunostaining
SOX2 Immunostaining
TRA-1-60 Immunostaining
TRA-1-81 Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: FOXA2, SOX17

Mesoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: HAND1, RUNX1

Ectoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: NR2F2, PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: mTeSR Plus - STEMCELL Technologies
  • Base Coat: Matrigel (hESC Qualified) - Corning

Passage Method: Enzyme-free cell dissociation