AIBNi013-A

Reviewed by Australian Stem Cell Registry

Alternative Names: hONS-derived Control iPSC; ONS-derived Control iPSC; Control HL iPSC

Details

Description

This induced pluripotent stem cell line was reprogrammed from a human olfactory neurosphere-derived cell line derived from a male donor using non-integrating sendai viral vectors. AIBNi013-A is described as having a normal karyotype. Expression of OCT4, SOX2, NANOG, TRA-1-60 and TRA-1-81 in undifferentiated cells, and directed differentiation to endoderm, mesoderm and ectoderm have been reported.

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma59684
Label: Mucosa of nose
Definition:
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2021 Reprogramming of human olfactory neurosphere-derived cells from olfactory mucosal biopsies of a control cohort

Line Custodianship

Cell Line Maintainer

Ernst Wolvetang

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia
View all cell lines from this group

Cell Line Producer

Australian Institute for Bioengineering and Nanotechnology

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia

Source

Donor Derived

Age: 25-29

Biological Sex: Male

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: EFO:0005705
Label: olfactory neurosphere cell line
Definition: Nasal biopsies were collected from volunteer donors as an outpatient procedure as described (Feron et al., 1998). This was done by a registered otolaryngologist via an endoscopic procedure to ensure biopsy samples were taken from the superior, posterior region of the nasal septum close to the cribriform plate. Pieces of olfactory mucosa were obtained which contain stem cells in the olfactory epithelium. The stem cell population derived from human olfactory mucosa has been described as ecto-mesenchymal (Delorme et al., 2010) because it has characteristics of both neural and mesenchymal stem cells, suggesting a neural crest origin.

Source Cell Origin: UBERON:0005386
Label: olfactory segment of nasal mucosa
Definition: That portion of the nasal mucosa containing the sensory endings for olfaction; the organ of smell[MESH].

Derivation Year: 2019

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Ethics

Ethics Number: ESK/01/11/HREC

Institution Human Research Ethics Council: Griffith University Human Research Ethics Committee

Approval Date: None

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XY

Karyotype Method: G-banding

Summary: Karyotyping occurred at a resolution of 400bphs. Analysis was performed from passage 5 onwards.

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
POU5F1 (OCT-4) Immunostaining
SOX2 Immunostaining
TRA-1-60 Immunostaining
TRA-1-81 Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: CXCR4, FOXA2, SOX17

Mesoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: CD34, HAND1, KDR, PDGFRA

Ectoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: NR2F2, OTX2, PAX6, VIM

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: mTeSR Plus - STEMCELL Technologies
  • Base Coat: Matrigel (hESC Qualified) - Corning

Passage Method: Enzyme-free cell dissociation