LEIi021-B

Reviewed by Australian Stem Cell Registry

Alternative Names: 1577ips9

Details

Description

This induced pluripotent stem cell line was reprogrammed from dermal fibroblasts using non-integrating episomal vectors. The donor of LEIi021-B is a male relative of a Retinitis Pigmentosa 11 patient and his unaffected/ non-penetrant mother who both carry a variant in the PRPF31 gene (NM_015629.3:c.1205C>A; LEIi008-A and LEIi009-A respectively). This variant was confirmed to be absent in LEIi021-B and its donor fibroblasts by Sanger sequencing. LEIi021-B is described as having a normal karyotype. Expression of OCT4, NANOG, SOX2 and SSEA-3 in undifferentiated cells, and directed differentiation to endoderm, mesoderm, ectoderm and retinal pigment epithelial cells have been reported.

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma7163
Label: Skin
Definition: Nonparenchymatous organ that consists of the epidermis, dermis and sets of hair and nails and which surrounds the entire body. Subdivisions of the skin surround various body parts; as a whole, the skin constitutes the external layer of the body. Examples: There is only one skin.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem Cell Research 2025 Derivation of two induced pluripotent stem cell lines from a healthy control subject

Line Custodianship

Cell Line Maintainer

Samuel McLenachan

Affiliated Institutions
  • Lions Eye Institute, Nedlands, Australia
View all cell lines from this group

Cell Line Producer

Lions Eye Institute

Affiliated Institutions
  • Lions Eye Institute, Nedlands, Australia

Source

Donor Derived

Age: 25-29

Biological Sex: Male

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: UBERON:0002067
Label: dermis
Definition: The dermis is a layer of skin between the epidermis (with which it makes up the skin) and subcutaneous tissues, and is composed of two layers, the papillary and reticular dermis[WP].

Derivation Year: 2019

Reprogramming Method

Vector Type: Not integrated

Vector: Episomal

Ethics

Ethics Number: RA/4/1/7916

Institution Human Research Ethics Council: University of Western Australia Human Research Ethics Committee

Approval Date: None

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 24

Karyotype: 46,XY

Karyotype Method: Molecular karyotyping by SNP array

Summary: Molecular karyotyping by SNP array (Illumina Infinium Human CytoSNP-12; resolution 500 kb).

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
NANOG Immunostaining
SOX2 Immunostaining
POU5F1 (OCT-4) RT-PCR
NANOG RT-PCR
SOX2 RT-PCR
SSEA-3 Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro spontaneous differentiation

Assessed by: In vitro spontaneous differentiation

Markers: FOXA2

Mesoderm

In vitro spontaneous differentiation

Assessed by: In vitro spontaneous differentiation

Markers: HAND1, SMN1, PAX3

Ectoderm

In vitro spontaneous differentiation

Assessed by: In vitro spontaneous differentiation

Markers: PAX6, OTX1, TUBB3

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 21%

Medium Items:

  • Base Medium: TeSR-E8 - STEMCELL Technologies
  • Base Coat: Geltrex - Thermo Fisher Scientific Inc.

Passage Method: Enzyme-free cell dissociation