UQi003-A

Reviewed by Australian Stem Cell Registry

Alternative Names: C7

Details

Description

This induced pluripotent stem cell line was reprogrammed from female fibroblasts using non-integrating self replicating RNA. UQi003-A is described as having a normal karyotype. Expression of SSEA-4 and NANOG and SOX2 in undifferentiated cells, and directed differentiation to endoderm, mesoderm have been reported.

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma7163
Label: Skin
Definition: Nonparenchymatous organ that consists of the epidermis, dermis and sets of hair and nails and which surrounds the entire body. Subdivisions of the skin surround various body parts; as a whole, the skin constitutes the external layer of the body. Examples: There is only one skin.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem Cell Research 2024 Generation of human induced pluripotent stem cell lines from sporadic, sporadic frontotemporal dementia, familial SOD1, and familial C9orf72 amyotrophic lateral sclerosis (ALS) patients.

Line Custodianship

Cell Line Maintainer

Shyuan Ngo

Affiliated Institutions
  • The University of Queensland, St Lucia, Australia
View all cell lines from this group

Cell Line Producer

The University of Queensland

Affiliated Institutions
  • The University of Queensland, St Lucia, Australia

Source

Donor Derived

Age: 65-69

Biological Sex: Female

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: UBERON:0002067
Label: dermis
Definition: The dermis is a layer of skin between the epidermis (with which it makes up the skin) and subcutaneous tissues, and is composed of two layers, the papillary and reticular dermis[WP].

Derivation Year: 2022

Reprogramming Method

Vector Type: Not integrated

Vector: Other

Ethics

Ethics Number: HREC/14/QRBW/495

Institution Human Research Ethics Council: Royal Brisbane and Women's Hospital

Approval Date: None

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XX

Karyotype Method: G-banding

Summary: Karyotyping occurred between passages 6 and 15 at a resolution of 500 bphs.

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
SSEA-4 Immunostaining
NANOG Immunostaining
SOX2 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: CXCR4, SOX17

Mesoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: NCAM1, TBXT

Ectoderm

In vitro directed differentiation

Assessed by: In vitro directed differentiation

Markers: NES, PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Matrigel - Corning

Passage Method: Enzyme-free cell dissociation