AIBNi017-A

Extracted from hPSCreg

SPGh1-S075mC2

Details

Tissue & Disease as reported

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2022 Generation of iPSC lines from hereditary spastic paraplegia 56 (SPG56) patients and family members carrying CYP2U1 mutations

Line Custodianship

Cell Line Maintainer

Australian Institute for Bioengineering and Nanotechnology

View all cell lines from this group

Cell Line Producer

Australian Institute for Bioengineering and Nanotechnology

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia

Source

Donor Derived

Age: Unknown

Biological Sex: Female

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000842
Label: mononuclear leukocyte
Definition: A leukocyte with a single non-segmented nucleus in the mature form.

Source Cell Origin: N/A

Derivation Year:

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Ethics

Ethics Number: 2019000159

Institution Human Research Ethics Council: Queensland Children's Health Services Human Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XX

Karyotype Method: Molecular karyotyping by SNP array

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
SOX2 Immunostaining
POU5F1 (OCT-4) Immunostaining
TRA 1-60 Immunostaining
TRA 1-81 Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Unavailable

Markers: CXCR4;SOX17

Mesoderm

In vitro directed differentiation

Assessed by: Unavailable

Markers: HAND1;PDGFRA

Ectoderm

In vitro directed differentiation

Assessed by: Unavailable

Markers: NR2F2;PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

Passage Method: Not specified