AIBNi013-A

Extracted from hPSCreg

ONS-derived Control iPSC, hONS-derived Control iPSC, Control HL iPSC

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma59684
Label: Mucosa of nose
Definition:
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2021 Reprogramming of human olfactory neurosphere-derived cells from olfactory mucosal biopsies of a control cohort
Stem Cell Research 2021 Reprogramming of human olfactory neurosphere-derived cells from olfactory mucosal biopsies of a control cohort.

Line Custodianship

Cell Line Maintainer

Wolvetang-AIBN

View all cell lines from this group

Cell Line Producer

Australian Institute for Bioengineering and Nanotechnology

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: N/A

Source Cell Origin: UBERON:0005386
Label: olfactory segment of nasal mucosa
Definition: That portion of the nasal mucosa containing the sensory endings for olfaction; the organ of smell[MESH].

Derivation Year: 2019

Reprogramming Method

Vector Type:

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: {{ details.non_integrated_vector.get_detected_reprogramming_vector_display }}

Ethics

Ethics Number: 2019000159

Institution Human Research Ethics Council: Queensland Children's Health Services Human Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 5

Karyotype: 46,XY

Karyotype Method: G-banding

Summary: Karyotyping occurred from passage 5 at a resolution of 400bphs.

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
OCT4 Immunostaining
SOX2 Immunostaining
TRA-1-60 Immunostaining
TRA-1-81 Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: RT-qPCR

Markers: CXCR4; FOXA2; SOX17

Mesoderm

In vitro directed differentiation

Assessed by: RT-qPCR

Markers: CD34; HAND1; KDR; PDGFRA

Ectoderm

In vitro directed differentiation

Assessed by: RT-qPCR

Markers: NR2F2; OTX2; PAX6; VIM

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: mTeSR Plus - STEMCELL Technologies
  • Base Coat: Matrigel (hESC Qualified) - Corning

Passage Method: Enzyme-free cell dissociation