MNZTASi021-A

Not yet reviewed by Australian Stem Cell Registry

MS_0021.1, MS_0021

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
medRxiv 2026 Multiple sclerosis iPSC derived-pericytes contract poorly but respond robustly to lesion-relevant environmental stimuli.
Stem cell research & therapy 2024 Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility

Line Custodianship

Cell Line Maintainer

Kaylene Young

View all cell lines from this group

Cell Line Producer

Menzies Institute for Medical Research

Affiliated Institutions
  • Menzies Institute for Medical Research, Hobart, Australia

Source

Donor Derived

Age: 75-79

Biological Sex: Male

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year:

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Ethics

Ethics Number: H0016915

Institution Human Research Ethics Council: University of Tasmania Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XY

Karyotype Method: G-banding

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
OCT4 RT-PCR
SOX2 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

None

Assessed by: Unavailable

Markers: HNF4A

Mesoderm

None

Assessed by: Unavailable

Markers: GATA4

Ectoderm

None

Assessed by: Unavailable

Markers: Nestin

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 20%

Medium Items:

  • Base Medium: mTeSR1 Plus - STEMCELL Technologies
  • Base Coat: Matrigel - Corning

Passage Method: Enzymatically