UOWi003-A

Not yet reviewed by Australian Stem Cell Registry

FAD1, 6848.2, iPSC 6848.2

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma37721
Label: Skin of arm
Definition:
Tissue for derived iPSC
Disease as reported (External Source)
alzheimer disease 3

Genetic Information

Genotype Locus
PSEN1
Polymorphism
Heterozygous: unspecified_reference_PSEN1:p.Ala246Glu

Associated Publications

Journal Year Article
Cells 2020 A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling
Stem Cell Research 2018 Generation and characterization of human induced pluripotent stem cell lines from a familial Alzheimer's disease PSEN1 A246E patient and a non-demented family member bearing wild-type PSEN1.
Stem cell research 2018 Generation and characterization of human induced pluripotent stem cell lines from a familial Alzheimer's disease PSEN1 A246E patient and a non-demented family member bearing wild-type PSEN1

Line Custodianship

Cell Line Maintainer

Ooi

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia
View all cell lines from this group

Cell Line Producer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: UBERON:0002097
Label: skin of body
Definition: The organ covering the body that consists of the dermis and epidermis.

Derivation Year: 2018

Ethics

Ethics Number: HE13/299

Institution Human Research Ethics Council: University of Wollongong Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XX

Karyotype Method: G-banding

Summary: Karyotyping occurred at a resolution of 400 bphs.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
SSEA-4 Immunostaining
NANOG RT-PCR
POU5F1 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

Classification Result
Self-renewal False
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency Characterisation

Endoderm

Unknown

Assessed by: Unavailable

Markers: None

Mesoderm

Unknown

Assessed by: Unavailable

Markers: None

Ectoderm

Unknown

Assessed by: Unavailable

Markers: None

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Matrigel - Corning
  • Base Medium: TeSR-E8 - STEMCELL Technologies

Passage Method: Not specified

External References

Source
Cells A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling
Stem Cell Research Generation and characterization of human induced pluripotent stem cell lines from a familial Alzheimer's disease PSEN1 A246E patient and a non-demented family member bearing wild-type PSEN1.
Stem cell research Generation and characterization of human induced pluripotent stem cell lines from a familial Alzheimer's disease PSEN1 A246E patient and a non-demented family member bearing wild-type PSEN1
hPSCreg UOWi003-A
Cellosaurus CVCL_VF29
Wikidata Q54991427
Phenomics Australia Phenomics Australia