UOWi009-A-1

Not yet reviewed by Australian Stem Cell Registry

MC6-3_#259, S290IC , MC_259

Details

Tissue & Disease as reported

Disease as reported (Genomic Modifications)

Genetic Information

Genotype Locus
PSEN1
Polymorphism
unspecified_reference_PSEN1:p.Ser290=
Modifications
Isogenic modification

Associated Publications

Journal Year Article
Frontiers in Cellular Neuroscience 2024 Alzheimer’s disease induced neurons bearing PSEN1 mutations exhibit reduced excitability.

Line Custodianship

Cell Line Maintainer

Dottori

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia
View all cell lines from this group

Cell Line Producer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: N/A

Source Cell Origin: N/A

Derivation Year:

Ethics

Ethics Number: Holding Entry-UOWi009-A-1

Institution Human Research Ethics Council: University of Wollongong Human Research Ethics Committee

Approval Date: To be verified

Modifications

Genomic Modifications

unspecified_reference_PSEN1:p.Ser290=

Correction of unspecified_reference_PSEN1:p.Ser290Cys variant present in the parental cell.

Cytoband: 14q24.2
Mutation Type: Isogenic modification
Delivery Method: CRISPR/Cas9

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XY

Karyotype Method: G-banding

Summary: Karyotyping occurred at a resolution of 400 bphs.

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
SSEA-4 Immunostaining
NANOG RT-PCR
POU5F1 (OCT-4) RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

Classification Result
Self-renewal False
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency Characterisation

Endoderm

Unknown

Assessed by: Unavailable

Markers: None

Mesoderm

Unknown

Assessed by: Unavailable

Markers: None

Ectoderm

Unknown

Assessed by: Unavailable

Markers: None

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: mTeSR - STEMCELL Technologies
  • Base Coat: Matrigel

Passage Method: Enzyme-free cell dissociation