UTSWi001-A-2

Not yet reviewed by Australian Stem Cell Registry

FA1-N

Details

Tissue & Disease as reported

Disease as reported (Genomic Modifications)

Genetic Information

Modifications
Transgene Expression

Associated Publications

No available publications

Line Custodianship

Cell Line Maintainer

Cell line maintainer not provided

Cell Line Producer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: N/A

Derivation Year:

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Ethics

Ethics Number: Holding Entry-UTSWi001-A

Institution Human Research Ethics Council: Holding Entry

Approval Date: None

Modifications

Genomic Modifications

Neurogenin 2

This line expresses Neurogenin 2 under a doxycycline inducible promoter. The line also contains a floxed Blasticidin resistance/nuclear mApple selection cassette.

Cytoband: 13q32.3
Mutation Type: Transgene Expression
Delivery Method: crispr

Quality Assurance

Genomic Characterisation

Passage Number:

Karyotype:

Karyotype Method:

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

No available data for this cell line.

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

Passage Method: Enzyme-free cell dissociation