LEIi005-A

Not yet reviewed by Australian Stem Cell Registry

Details

Tissue & Disease as reported

Disease as reported (Donor)
retinitis pigmentosa

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Molecular genetics & genomic medicine 2020 Characterization of CRB1 splicing in retinal organoids derived from a patient with adult-onset rod-cone dystrophy caused by the c.1892A>G and c.2548G>A variants

Line Custodianship

Cell Line Maintainer

Lions Eye Institute

Affiliated Institutions
  • Lions Eye Institute, Nedlands, Australia
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Cell Line Producer

Lions Eye Institute

Affiliated Institutions
  • Lions Eye Institute, Nedlands, Australia

Source

Donor Derived

Age: Unknown

Biological Sex: Female

Disease: MONDO:0019200
Label: retinitis pigmentosa
Definition: Retinitis pigmentosa (RP) is an inherited retinal dystrophy leading to progressive loss of the photoreceptors and retinal pigment epithelium and resulting in blindness usually after several decades.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: N/A

Derivation Year:

Reprogramming Method

Vector Type: Not integrated

Vector: Episomal

Ethics

Ethics Number: RA/4/1/7916

Institution Human Research Ethics Council: University of Western Australia Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 45

Karyotype: 46+XX

Karyotype Method: Other

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

Marker Method
NANOG RT-PCR
NANOG Immunostaining
POU5F1 (OCT-4) Immunostaining
POU5F1 (OCT-4) RT-PCR
SSEA-3 Immunostaining
SOX2 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 20%

Medium Items:

Passage Method: Enzyme-free cell dissociation