VCCRIi002-A

Reviewed and Updated by Australian Stem Cell Registry

069

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
idiopathic spontaneous coronary artery dissection fibromuscular dysplasia

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2023 Generation of induced pluripotent stem cell lines from a sister pair who suffered post-partum or recurrent Spontaneous Coronary Artery Dissections

Line Custodianship

Cell Line Maintainer

Graham

Affiliated Institutions
  • Victor Chang Cardiac Research Institute, Sydney, Australia
View all cell lines from this group

Cell Line Producer

Victor Chang Cardiac Research Institute

Affiliated Institutions
  • Victor Chang Cardiac Research Institute, Sydney, Australia

Source

Donor Derived

Age: 40-44

Biological Sex: Female

Disease: MONDO:0007385
Label: idiopathic spontaneous coronary artery dissection
Definition:

Disease: EFO:1000938
Label: fibromuscular dysplasia
Definition: A disorder characterized by fibrous thickening of the arterial wall resulting in narrowing of the arterial lumen. It most often affects the renal artery and less often the carotid artery and abdominal arteries. It can cause hypertension and aneurysm formation.| An idiopathic, segmental, nonatheromatous disease of the musculature of arterial walls, leading to STENOSIS of small and medium-sized arteries. There is true proliferation of SMOOTH MUSCLE CELLS and fibrous tissue. Fibromuscular dysplasia lesions are smooth stenosis and occur most often in the renal and carotid arteries. They may also occur in other peripheral arteries of the extremity.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2020

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: No

Ethics

Ethics Number: HREC/16/SVH/338

Institution Human Research Ethics Council: St Vincent’s Hospital Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 20

Karyotype: 46,XX

Karyotype Method: G-banding

Summary: Karyotyping occurred at a resolution of 400 bphs (15 cells).

STR analysis Results:

Performed but not available.

Genome wide or Functional Assay:

Microbiology and Virology Screening

Disease Result
HIV 1 Negative
HIV 2 Negative
HEP B Negative
HEP C Negative
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
SSEA-4 Immunostaining
NANOG Immunostaining
POU5F1 (OCT-4) Immunostaining
TRA-1-60 Immunostaining

Scorecard Results

Undifferentiated Cells

Classification Result
Self-renewal True
Endoderm False
Mesoderm False
Ectoderm False

Pluripotency

Classification Result
Self-renewal False
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: SOX17

Mesoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: Brachyury

Ectoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 18%

Medium Items:

  • Base Medium: mTeSR Plus
  • Base Coat: Matrigel

Passage Method: Enzyme-free cell dissociation