MONUi003-B

Not yet reviewed by Australian Stem Cell Registry

213.2, 86203213.2

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
autism spectrum disorder

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2023 Generation of induced pluripotent stem cell lines from three individuals with autism spectrum disorder
Stem Cell Research 2023 Generation of induced pluripotent stem cell lines from three individuals with autism spectrum disorder.

Line Custodianship

Cell Line Maintainer

Bellgrove

View all cell lines from this group

Cell Line Producer

Monash University

Affiliated Institutions
  • Monash University, Leeds, United Kingdom

Source

Donor Derived

Age: 10-14

Biological Sex: Male

Disease: MONDO:0005258
Label: autism spectrum disorder
Definition: A spectrum of developmental disorders that includes autism, and Asperger syndrome. Signs and symptoms include poor communication skills, defective social interactions, and repetitive behaviors.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2023

Reprogramming Method

Vector Type: Not integrated

Vector: Episomal

Kit: pEV-SFFV-MYC

Detected: Unknown

Ethics

Ethics Number: 25688

Institution Human Research Ethics Council: Monash University Human Research Ethics Committee (MUHREC)

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 10

Karyotype: arr(1-22)x2,(XY)x1

Karyotype Method: Molecular karyotyping by SNP array

Summary: Molecular karyotyping by SNP array (Illumina Global Screening Array-24 v3.0; resolution 0.5Mb). SNPduo comparison to parental PBMCs demonstrated >99.9% concordance at 650,000 SNP markers.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
POU5F1 (OCT-4) Flow cytometry
SSEA-4 Immunostaining
SSEA-4 Flow cytometry
TRA 1-60 Flow cytometry
TRA 1-81 Flow cytometry

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: FOXA2;SOX17

Mesoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: Brachyury;SMA

Ectoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: NEUN;MAP2

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Matrigel
  • Base Medium: Essential 8 Medium - Invitrogen

Passage Method: Enzyme-free cell dissociation