UOWi008-A

Not yet reviewed by Australian Stem Cell Registry

RBC-10, , C-10, ALS/FTD1

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma7163
Label: Skin
Definition: Nonparenchymatous organ that consists of the epidermis, dermis and sets of hair and nails and which surrounds the entire body. Subdivisions of the skin surround various body parts; as a whole, the skin constitutes the external layer of the body. Examples: There is only one skin.
Tissue for derived iPSC
Disease as reported (Donor)
frontotemporal dementia and/or amyotrophic lateral sclerosis 1

Genetic Information

Genotype Locus
C9orf72
Polymorphism
C9orf72 repeat expansion

Associated Publications

Journal Year Article
Cells 2020 A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling
Cells 2020 A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling.

Line Custodianship

Cell Line Maintainer

Ooi

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia
View all cell lines from this group

Cell Line Producer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia

Source

Donor Derived

Age: 65-69

Biological Sex: Female

Disease: MONDO:0007105
Label: frontotemporal dementia and/or amyotrophic lateral sclerosis 1
Definition: Any frontotemporal dementia with motor neuron disease in which the cause of the disease is a mutation in the C9orf72 gene.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: UBERON:0002067
Label: dermis
Definition: The dermis is a layer of skin between the epidermis (with which it makes up the skin) and subcutaneous tissues, and is composed of two layers, the papillary and reticular dermis[WP].

Derivation Year:

Ethics

Ethics Number: HE13/299

Institution Human Research Ethics Council: University of Wollongong Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XX

Karyotype Method: G-banding

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
SSEA-4 Immunostaining
TRA-1-60 Immunostaining
NANOG RT-PCR
POU5F1 (OCT-4) RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

Classification Result
Self-renewal False
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency Characterisation

Endoderm

Unknown

Assessed by: Unavailable

Markers: None

Mesoderm

Unknown

Assessed by: Unavailable

Markers: None

Ectoderm

Unknown

Assessed by: Unavailable

Markers: None

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Matrigel
  • Base Medium: TeSR-E8

Passage Method: Enzymatically

External References

Source
Cells A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling
Cells A Simple Differentiation Protocol for Generation of Induced Pluripotent Stem Cell-Derived Basal Forebrain-Like Cholinergic Neurons for Alzheimer's Disease and Frontotemporal Dementia Disease Modeling.
hPSCreg UOWi008-A
Cellosaurus CVCL_VP86
Wikidata Q98134331
Phenomics Australia Phenomics Australia