UOWi007-A

Not yet reviewed by Australian Stem Cell Registry

MQ54-2

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma7163
Label: Skin
Definition: Nonparenchymatous organ that consists of the epidermis, dermis and sets of hair and nails and which surrounds the entire body. Subdivisions of the skin surround various body parts; as a whole, the skin constitutes the external layer of the body. Examples: There is only one skin.
Tissue for derived iPSC
Disease as reported (Donor)
amyotrophic lateral sclerosis

Genetic Information

Genotype Locus
SOD1
Polymorphism
Heterozygous: unspecified_reference_SOD1:p.Glu101Gly

Associated Publications

Journal Year Article
Stem Cell Research 2020 The mRNA-based reprogramming of fibroblasts from a SOD1
Stem cell research 2020 The mRNA-based reprogramming of fibroblasts from a SOD1(E101G) familial amyotrophic lateral sclerosis patient to induced pluripotent stem cell line UOWi007

Line Custodianship

Cell Line Maintainer

Ooi

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia
View all cell lines from this group

Cell Line Producer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia

Source

Donor Derived

Age: 40-44

Biological Sex: Male

Disease: MONDO:0004976
Label: amyotrophic lateral sclerosis
Definition: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease characterized by progressive muscular paralysis reflecting degeneration of motor neurons in the primary motor cortex, corticospinal tracts, brainstem and spinal cord.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: UBERON:0002067
Label: dermis
Definition: The dermis is a layer of skin between the epidermis (with which it makes up the skin) and subcutaneous tissues, and is composed of two layers, the papillary and reticular dermis[WP].

Derivation Year: 2018

Ethics

Ethics Number: HE13/272

Institution Human Research Ethics Council: University of Wollongong Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 24

Karyotype: 46,XY

Karyotype Method: G-banding

Summary: Karyotyping occurred at a resolution of 400bphs (15 metaphase spreads).

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
SSEA-4 Immunostaining
TRA-1-60 Immunostaining
POU5F1 RT-PCR
NANOG RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

Classification Result
Self-renewal False
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency Characterisation

Endoderm

Unknown

Assessed by: Unavailable

Markers: None

Mesoderm

Unknown

Assessed by: Unavailable

Markers: None

Ectoderm

Unknown

Assessed by: Unavailable

Markers: None

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: TeSR-E8 - STEMCELL Technologies
  • Base Coat: Matrigel - Corning

Passage Method: Enzymatically