MCRIi022-A

Not yet reviewed by Australian Stem Cell Registry

NF1_093.2

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
neurofibromatosis type 1

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2020 Generation of four iPSC lines from Neurofibromatosis Type 1 patients
Stem Cell Research 2020 Generation of four iPSC lines from Neurofibromatosis Type 1 patients.

Line Custodianship

Cell Line Maintainer

Lockhart

Affiliated Institutions
  • Murdoch Children's Research Institute, Melbourne, Australia
View all cell lines from this group

Cell Line Producer

Murdoch Children's Research Institute

Affiliated Institutions
  • Murdoch Children's Research Institute, Melbourne, Australia

Source

Donor Derived

Age: 10-14

Biological Sex: Male

Disease: MONDO:0018975
Label: neurofibromatosis type 1
Definition: A clinically heterogeneous, neurocutaneous genetic disorder characterized by cafe-au-lait spots, iris Lisch nodules, axillary and inguinal freckling, and multiple neurofibromas.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2020

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: Unknown

Ethics

Ethics Number: 35118

Institution Human Research Ethics Council: Royal Children's Hospital Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 3

Karyotype: arr(1-22)x2,(XY)x1

Karyotype Method: Molecular karyotyping by SNP array

Summary: Molecular karyotyping by SNP array (Illumina Infinium GSA-24 v2.0). SNPduo comparison to parental cell (>99.9% identity).

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
POU5F1 (OCT-4) Immunostaining
CD9 Flow cytometry
EPCAM Flow cytometry
SSEA-4 Flow cytometry
TRA-1-81 Flow cytometry

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: SOX17

Mesoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: SMA

Ectoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: MAP2

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 10%

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Vitronectin - STEMCELL Technologies
  • Base Medium: Essential 8 Medium - Thermo Fisher Scientific Inc.

Passage Method: Not specified