MNZTASi019-A

Not yet reviewed by Australian Stem Cell Registry

MS_0019

Details

Tissue & Disease as reported

Genetic Information

No genetic information available for this cell line

Associated Publications

Line Custodianship

Cell Line Maintainer

Menzies Institute for Medical Research

Affiliated Institutions
  • Menzies Institute for Medical Research, Hobart, Australia
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Cell Line Producer

Menzies Institute for Medical Research

Affiliated Institutions
  • Menzies Institute for Medical Research, Hobart, Australia

Source

Donor Derived

Age: Unknown

Biological Sex: Female

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000842
Label: mononuclear leukocyte
Definition: A leukocyte with a single non-segmented nucleus in the mature form.

Source Cell Origin: UBERON:0003822
Label: forelimb stylopod
Definition: The part of the forelimb between pectoral region and the elbow, corresponding to the humerus.

Derivation Year:

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Ethics

Ethics Number: H0016915

Institution Human Research Ethics Council: University of Tasmania Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: female

Karyotype Method: Other

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
OCT4 RT-PCR
SOX2 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

None

Assessed by: Unavailable

Markers: HNF4A

Mesoderm

None

Assessed by: Unavailable

Markers: GATA4

Ectoderm

None

Assessed by: Unavailable

Markers: Nestin

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 20%

Medium Items:

Passage Method: Enzymatically