MNZTASi005-A

Not yet reviewed by Australian Stem Cell Registry

MS_0006

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
relapsing-remitting multiple sclerosis

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2022 Generation and characterisation of four multiple sclerosis iPSC lines from a single family
Stem Cell Research 2022 Generation and characterisation of four multiple sclerosis iPSC lines from a single family.

Line Custodianship

Cell Line Maintainer

Young

View all cell lines from this group

Cell Line Producer

Menzies Institute for Medical Research

Affiliated Institutions
  • Menzies Institute for Medical Research, Hobart, Australia

Source

Donor Derived

Age: Unknown

Biological Sex: Male

Disease: MONDO:0005314
Label: relapsing-remitting multiple sclerosis
Definition: The most common clinical variant of multiple sclerosis, characterized by recurrent acute exacerbations of neurologic dysfunction followed by partial or complete recovery. Common clinical manifestations include loss of visual (see optic neuritis), motor, sensory, or bladder function. Acute episodes of demyelination may occur at any site in the central nervous system, and commonly involve the optic nerves, spinal cord, brain stem, and cerebellum. (Adams et al., Principles of Neurology, 6th ed, pp903-914)

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2021

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: No

Ethics

Ethics Number: H0016915

Institution Human Research Ethics Council: University of Tasmania Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XY

Karyotype Method: G-banding

Summary: Karyotyping (20 metaphases) occurred at a resolution of 550 bphs.

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
HIV 1 Negative
HIV 2 Negative
HEP B Negative
HEP C Negative
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
OCT4 RT-PCR
SOX2 RT-PCR
Nanog RT-PCR
POU5F1 (OCT-4) Immunostaining
POU5F1 (OCT-4) Flow cytometry
NANOG RT-PCR
POU5F1 (OCT-4) RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: AFP

Mesoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: GATA4

Ectoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: NES

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 20%

Medium Items:

  • Base Medium: mTeSR Plus - STEMCELL Technologies
  • Base Coat: Matrigel

Passage Method: Enzymatically