MCRIi001-A-OI26, PB001-OI26
Bateman
Murdoch Children's Research Institute
Source Cell Type:
CL:0000765
Label: erythroblast
Definition:
A nucleated precursor of an erythrocyte that lacks hematopoietic lineage markers.
Source Cell Origin:
UBERON:0000178
Label: blood
Definition:
A fluid that is composed of blood plasma and erythrocytes.
Derivation Year:
Ethics Number: 35121 A
Institution Human Research Ethics Council: RCH HUMAN RESEARCH ETHICS COMMITTEE
Approval Date: To be verified
A group of usually autosomal dominant inherited disorders characterized by defective synthesis of collagen type I resulting in defective collagen formation. It is characterized by brittle and easily fractured bones.
Introduction of heterozygous COL1A1:c.3969_3970insT variant in exon 49 leading to a frameshift and insertion of a premature stop codon. Synonymous changes were also introduced.
Passage Number: 2
Karyotype: arr(1-22)x2,(XY)x1
Karyotype Method: Molecular karyotyping by SNP array
Summary: Molecular karyotyping by SNP array (Illumina Infinium CoreExome-24 v1.1; resolution 0.5 Mb). SNPduo comparison to parental cell (>99.9% identity).
| Disease | Result |
|---|---|
| Mycoplasma | Negative |
| Marker | Method |
|---|---|
| NANOG | Immunostaining |
| POU5F1 (OCT-4) | Immunostaining |
| CD9 | Flow cytometry |
| SSEA-3 | Flow cytometry |
| TRA-1-81 | Flow cytometry |
No available data for this cell line.
No available data for this cell line.
In vitro directed differentiation
Assessed by: Flow cytometry
Markers: CXCR4;EPCAM
In vitro directed differentiation
Assessed by: Flow cytometry
Markers: CD45
In vitro directed differentiation
Assessed by: Immunostaining
Markers: NES;PAX6
CO2 concentration: 5%
O2 concentration: 95%
Medium Items:
Passage Method: Enzyme-free cell dissociation