WAe009-A-W

Not yet reviewed by Australian Stem Cell Registry

H9 NGN2 B6

Details

Tissue & Disease as reported

Disease as reported (Genomic Modifications)

Genetic Information

Modifications
Transgene Expression

Associated Publications

Journal Year Article
Stem cell research 2024 Generation of a gene-edited H9 embryonic stem cell line carrying a DOX-inducible NGN2 expression cassette in the CLYBL locus

Line Custodianship

Cell Line Maintainer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia
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Cell Line Producer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: N/A

Source Cell Origin: N/A

Derivation Year:

Ethics

Ethics Number: Holding Entry-WAe009-A

Institution Human Research Ethics Council: Holding Entry

Approval Date: None

Modifications

Genomic Modifications

This line expresses Neurogenin 2 under a doxycycline inducible promoter. The line also contains a floxed Blasticidin resistance/nuclear mApple selection cassette.

Cytoband: 13q32.3
Mutation Type: Transgene Expression
Delivery Method: crispr

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46, XX

Karyotype Method: G-banding

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

No available data for this cell line.

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

Passage Method: Not specified