hONS-derived A-T iPSC, A-T HL iPSC, ONS-derived A-T iPSC
Ernst Wolvetang
Australian Institute for Bioengineering and Nanotechnology
Griffith Institute for Drug Discovery Neuro Bank
Name: Human olfactory neurosphere derived cells
Accession Identifier: 906110003
Disease: ataxia telangiectasia
Source Cell Type:
EFO:0005705
Label: olfactory neurosphere cell line
Definition:
Nasal biopsies were collected from volunteer donors as an outpatient procedure as described (Feron et al., 1998). This was done by a registered otolaryngologist via an endoscopic procedure to ensure biopsy samples were taken from the superior, posterior region of the nasal septum close to the cribriform plate. Pieces of olfactory mucosa were obtained which contain stem cells in the olfactory epithelium. The stem cell population derived from human olfactory mucosa has been described as ecto-mesenchymal (Delorme et al., 2010) because it has characteristics of both neural and mesenchymal stem cells, suggesting a neural crest origin.
Source Cell Origin:
UBERON:0005386
Label: olfactory segment of nasal mucosa
Definition:
That portion of the nasal mucosa containing the sensory endings for olfaction; the organ of smell[MESH].
Derivation Year: 2018
Vector Type:
Vector: Other
Kit: [kit] ReproRNA-OKSGM
Detected: Unknown
Ethics Number: HREC/09/QRCH/103
Institution Human Research Ethics Council: Queensland Children's Health Services Human Ethics Committee
Approval Date: To be verified
No genomic modifications available for this line.
Passage Number: 5
Karyotype: 46,XX
Karyotype Method: G-banding
Summary: Karyotyping occurred from passage 5 at a resolution of 500bphs.
STR analysis Results:
Performed but not available.| Disease | Result |
|---|---|
| Mycoplasma | Negative |
| Marker | Method |
|---|---|
| NANOG | Immunostaining |
| POU5F1 (OCT-4) | Immunostaining |
| SOX2 | Immunostaining |
| TRA-1-60 | Immunostaining |
| TRA-1-81 | Immunostaining |
No available data for this cell line.
No available data for this cell line.
In vitro directed differentiation
Assessed by: RT-PCR
Markers: CXCR4;FOXA2;SOX17
In vitro directed differentiation
Assessed by: RT-PCR
Markers: CD34;HAND1;KDR;PDGFRA
In vitro directed differentiation
Assessed by: RT-PCR
Markers: NR2F2;OTX2;PAX6;VIM
CO2 concentration: 5%
O2 concentration: Unavailable
Medium Items:
Passage Method: Enzyme-free cell dissociation