AIBNi014-A

Not yet reviewed by Australian Stem Cell Registry

A-T HL iPSC, ONS-derived A-T iPSC, hONS-derived A-T iPSC

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma59684
Label: Mucosa of nose
Definition:
Tissue for derived iPSC
Disease as reported (External Source)
ataxia telangiectasia

Genetic Information

Genotype Locus
ATM
Polymorphism
Homozygous: NM_000051.3:c.1236-3dupT; Heterozygous: NM_000051.3:c.6404_6405insTT(p.Arg2136fs)

Associated Publications

Journal Year Article
Aging cell 2021 Inhibition of the cGAS-STING pathway ameliorates the premature senescence hallmarks of Ataxia-Telangiectasia brain organoids
Stem cell research 2021 Ataxia Telangiectasia iPSC line generated from a patient olfactory biopsy identifies novel disease-causing mutations
Stem Cell Research 2021 Ataxia Telangiectasia iPSC line generated from a patient olfactory biopsy identifies novel disease-causing mutations.
Aging Cell 2021 Inhibition of the cGAS-STING pathway ameliorates the premature senescence hallmarks of Ataxia-Telangiectasia brain organoids.

Line Custodianship

Cell Line Maintainer

Wolvetang

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia
View all cell lines from this group

Cell Line Producer

Australian Institute for Bioengineering and Nanotechnology

Affiliated Institutions
  • Australian Institute for Bioengineering and Nanotechnology, Brisbane, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: N/A

Source Cell Origin: UBERON:0005386
Label: olfactory segment of nasal mucosa
Definition: That portion of the nasal mucosa containing the sensory endings for olfaction; the organ of smell[MESH].

Derivation Year: 2018

Reprogramming Method

Vector Type:

Vector: Other

Kit: [kit] ReproRNA-OKSGM

Detected: Unknown

Ethics

Ethics Number: HREC/09/QRCH/103

Institution Human Research Ethics Council: Queensland Children's Health Services Human Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 5

Karyotype: 46,XX

Karyotype Method: G-banding

Summary: Karyotyping occurred from passage 5 at a resolution of 500bphs.

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
POU5F1 (OCT-4) Immunostaining
SOX2 Immunostaining
TRA-1-60 Immunostaining
TRA-1-81 Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: RT-PCR

Markers: CXCR4;FOXA2;SOX17

Mesoderm

In vitro directed differentiation

Assessed by: RT-PCR

Markers: CD34;HAND1;KDR;PDGFRA

Ectoderm

In vitro directed differentiation

Assessed by: RT-PCR

Markers: NR2F2;OTX2;PAX6;VIM

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: mTeSR Plus - STEMCELL Technologies
  • Base Coat: Matrigel (hESC Qualified) - Corning

Passage Method: Enzyme-free cell dissociation

External References

Source
Aging cell Inhibition of the cGAS-STING pathway ameliorates the premature senescence hallmarks of Ataxia-Telangiectasia brain organoids
Stem cell research Ataxia Telangiectasia iPSC line generated from a patient olfactory biopsy identifies novel disease-causing mutations
Stem Cell Research Ataxia Telangiectasia iPSC line generated from a patient olfactory biopsy identifies novel disease-causing mutations.
Aging Cell Inhibition of the cGAS-STING pathway ameliorates the premature senescence hallmarks of Ataxia-Telangiectasia brain organoids.
hPSCreg AIBNi014-A
Cellosaurus CVCL_B3PY
Wikidata Q110432574
Phenomics Australia Phenomics Australia