MCRIi005-A

Not yet reviewed by Australian Stem Cell Registry

PB005

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2019 Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

Line Custodianship

Cell Line Maintainer

Vlahos

Affiliated Institutions
  • Murdoch Children's Research Institute, Melbourne, Australia
View all cell lines from this group

Cell Line Producer

Murdoch Children's Research Institute

Affiliated Institutions
  • Murdoch Children's Research Institute, Melbourne, Australia

Source

Donor Derived

Age: 45-49

Biological Sex: Female

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2016

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: No

Ethics

Ethics Number: 35121 A

Institution Human Research Ethics Council: RCH HUMAN RESEARCH ETHICS COMMITTEE

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: arr(1-22,X)x2

Karyotype Method: Molecular karyotyping by SNP array

Summary: Molecular karyotyping by SNP array (Illumina Human CytoSNP-12; resolution 0.5 Mb). SNPduo comparison to parental cell (>99.9% identity).

Microbiology and Virology Screening

Disease Result
HIV 1 Negative
HIV 2 Negative
HEP B Negative
HEP C Negative
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
SSEA-4 Flow cytometry
TRA 1-81 Flow cytometry
CD9 Flow cytometry

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Flow cytometry

Markers: CXCR4;EPCAM;KIT

Mesoderm

In vitro directed differentiation

Assessed by: Flow cytometry

Markers: CD13;EPCAM

Ectoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: MAP2

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

  • Base Medium: DMEM/F12
  • Main Protein: Knock-out Serum Replacement
  • Supplement: FGF2
  • Supplement: GlutaMAX
  • Supplement: 2-ME
  • Supplement: NEAA

Passage Method: Not specified