CIAUi002-C

Not yet reviewed by Australian Stem Cell Registry

UBC3 M1

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
left bundle branch block nonischemic cardiomyopathy

Genetic Information

Genotype Locus
SCN5A
Polymorphism
Heterozygous: NM_000335.5:c.392+3A>G

Associated Publications

Journal Year Article
Stem cell research 2023 Generation of an induced pluripotent stem cell line from a patient with conduction disease and recurrent ventricular fibrillation with a sodium voltage-gated channel alpha subunit 5 (SCN5A) gene c.392 + 3A > G splice-site variant

Line Custodianship

Cell Line Maintainer

Semsarian

View all cell lines from this group

Cell Line Producer

Centenary Institute of Cancer Medicine and Cell Biology

Affiliated Institutions
  • Centenary Institute of Cancer Medicine and Cell Biology, Camperdown, Australia

Source

Donor Derived

Age: 30-34

Biological Sex: Female

Disease: HP:0011713
Label: Left bundle branch block
Definition: A conduction block of the left branch of the bundle of His. This manifests as a generalized disturbance of QRS morphology on EKG.

Disease: EFO:0009881
Label: nonischemic cardiomyopathy
Definition: Forms of cardiomyopathy that are not related to known coronary artery disease.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2021

Reprogramming Method

Vector Type: Not integrated

Vector: Episomal

Kit: [kit] Epi5 Episomal iPSC Reprogramming Kit

Detected: No

Ethics

Ethics Number: X19-0108 and 2019/ETH00461

Institution Human Research Ethics Council: Ethics Review Committee, Sydney Local Health District

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: Normal

Karyotype Method: Other

Summary: Molecular karyotyping by SNP array (Illumina Infinium Global Screening Array 24, v1.0; resolution 0.50 Mb).

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
SSEA-4 Immunostaining
TRA 1-60 Immunostaining
NANOG RT-PCR
SOX2 RT-PCR
SOX2 Immunostaining
OCT4 Immunostaining
OCT4 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: CXCR4;SOX17

Mesoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: NKX2.5;TNNT2

Ectoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: NES;PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: mTeSR Plus - STEMCELL Technologies
  • Base Coat: Matrigel (hESC Qualified) - Corning

Passage Method: Enzyme-free cell dissociation