MCRIi001-A

Not yet reviewed by Australian Stem Cell Registry

PB001

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2024 Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing
ACS Pharmacology & Translational Science 2024 10.1021/acsptsci.3c00324
Biomaterials Research 2023 10.1186/s40824-023-00382-x
Karbala International Journal of Modern Science 2023 10.33640/2405-609x.3330
Frontiers in Bioscience-Landmark 2023 10.31083/j.fbl2812336
Transboundary and Emerging Diseases 2023 10.1155/2023/9098445
Methods in molecular biology (Clifton, N.J.) 2023 Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells
Proceedings of the National Academy of Sciences of the United States of America 2023 Modeling human skeletal development using human pluripotent stem cells
Stem Cell Reviews and Reports 2023 10.1007/s12015-023-10585-3
Seminars in cell & developmental biology 2022 Developmental principles informing human pluripotent stem cell differentiation to cartilage and bone
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2022 Curative Cell and Gene Therapy for Osteogenesis Imperfecta
Circulation 2021 Sex-Specific Control of Human Heart Maturation by the Progesterone Receptor
Biomolecules 2021 Potential of Induced Pluripotent Stem Cells for Use in Gene Therapy: History, Molecular Bases, and Medical Perspectives
Biomolecules 2021 Osteogenesis Imperfecta: Current and Prospective Therapies
Computational and structural biotechnology journal 2020 Probing pluripotency gene regulatory networks with quantitative live cell imaging
Frontiers in cellular neuroscience 2020 The Use of Induced Pluripotent Stem Cells as a Model for Developmental Eye Disorders
Frontiers in endocrinology 2020 Genomic Medicine: Lessons Learned From Monogenic and Complex Bone Disorders
Theranostics 2020 Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases
Stem cell research 2020 Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing
Stem cell research 2020 CRISPR/Cas9 gene editing of a SOX9 reporter human iPSC line to produce two TRPV4 patient heterozygous missense mutant iPSC lines, MCRIi001-A-3 (TRPV4 p.F273L) and MCRIi001-A-4 (TRPV4 p.P799L)
Stem cell research 2020 Generation of a heterozygous COL2A1 (p.R989C) spondyloepiphyseal dysplasia congenita mutation iPSC line, MCRIi001-B, using CRISPR/Cas9 gene editing
Stem cell research 2019 Generation of a heterozygous COL1A1 (c.3969_3970insT) osteogenesis imperfecta mutation human iPSC line, MCRIi001-A-1, using CRISPR/Cas9 editing
Stem cell research 2019 Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

Line Custodianship

Cell Line Maintainer

Vlahos

Affiliated Institutions
  • Murdoch Children's Research Institute, Melbourne, Australia
View all cell lines from this group

Cell Line Producer

Murdoch Children's Research Institute

Affiliated Institutions
  • Murdoch Children's Research Institute, Melbourne, Australia

Source

Donor Derived

Age: 60-64

Biological Sex: Male

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2016

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: No

Ethics

Ethics Number: 35121 A

Institution Human Research Ethics Council: RCH HUMAN RESEARCH ETHICS COMMITTEE

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: arr(1-22)x2,(XY)x1

Karyotype Method: Molecular karyotyping by SNP array

Summary: Molecular karyotyping by SNP array (Illumina Human CytoSNP-12; resolution 0.5 Mb). SNPduo comparison to parental cell (>99.9% identity).

Microbiology and Virology Screening

Disease Result
HIV 1 Negative
HIV 2 Negative
HEP B Negative
HEP C Negative
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
SSEA-4 Flow cytometry
TRA 1-81 Flow cytometry
CD9 Flow cytometry

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Flow cytometry

Markers: CXCR4;EPCAM;KIT

Mesoderm

In vitro directed differentiation

Assessed by: Flow cytometry

Markers: CD13;EPCAM

Ectoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: MAP2

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

  • Base Medium: DMEM/F12
  • Main Protein: Knock-out Serum Replacement
  • Supplement: FGF2
  • Supplement: GlutaMAX
  • Supplement: 2-ME
  • Supplement: NEAA

Passage Method: Not specified

External References

Source
Stem cell research Generation of a human ACTA1-tdTomato reporter iPSC line using CRISPR/Cas9 editing
ACS Pharmacology & Translational Science 10.1021/acsptsci.3c00324
Biomaterials Research 10.1186/s40824-023-00382-x
Karbala International Journal of Modern Science 10.33640/2405-609x.3330
Frontiers in Bioscience-Landmark 10.31083/j.fbl2812336
Transboundary and Emerging Diseases 10.1155/2023/9098445
Methods in molecular biology (Clifton, N.J.) Chondrogenic Differentiation of Human-Induced Pluripotent Stem Cells
Proceedings of the National Academy of Sciences of the United States of America Modeling human skeletal development using human pluripotent stem cells
Stem Cell Reviews and Reports 10.1007/s12015-023-10585-3
Seminars in cell & developmental biology Developmental principles informing human pluripotent stem cell differentiation to cartilage and bone
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research Curative Cell and Gene Therapy for Osteogenesis Imperfecta
Circulation Sex-Specific Control of Human Heart Maturation by the Progesterone Receptor
Biomolecules Potential of Induced Pluripotent Stem Cells for Use in Gene Therapy: History, Molecular Bases, and Medical Perspectives
Biomolecules Osteogenesis Imperfecta: Current and Prospective Therapies
Computational and structural biotechnology journal Probing pluripotency gene regulatory networks with quantitative live cell imaging
Frontiers in cellular neuroscience The Use of Induced Pluripotent Stem Cells as a Model for Developmental Eye Disorders
Frontiers in endocrinology Genomic Medicine: Lessons Learned From Monogenic and Complex Bone Disorders
Theranostics Advances in CRISPR/Cas-based Gene Therapy in Human Genetic Diseases
Stem cell research Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing
Stem cell research CRISPR/Cas9 gene editing of a SOX9 reporter human iPSC line to produce two TRPV4 patient heterozygous missense mutant iPSC lines, MCRIi001-A-3 (TRPV4 p.F273L) and MCRIi001-A-4 (TRPV4 p.P799L)
Stem cell research Generation of a heterozygous COL2A1 (p.R989C) spondyloepiphyseal dysplasia congenita mutation iPSC line, MCRIi001-B, using CRISPR/Cas9 gene editing
Stem cell research Generation of a heterozygous COL1A1 (c.3969_3970insT) osteogenesis imperfecta mutation human iPSC line, MCRIi001-A-1, using CRISPR/Cas9 editing
Stem cell research Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults
hPSCreg MCRIi001-A
Cellosaurus CVCL_UK82
BioSamples SAMEA4966484
Wikidata Q95986990
Phenomics Australia Phenomics Australia