MNZTASi001-A

Not yet reviewed by Australian Stem Cell Registry

MS_0004.2 , 2125-2, MS_0004, TOB-02125

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
primary progressive multiple sclerosis

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research & therapy 2024 Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility
Frontiers in Immunology 2024 10.3389/fimmu.2024.1349138
Stem cell research 2022 Generation and characterisation of four multiple sclerosis iPSC lines from a single family
Stem cell research 2021 Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis
Stem Cell Research 2021 Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis.

Line Custodianship

Cell Line Maintainer

Young

View all cell lines from this group

Cell Line Producer

Menzies Institute for Medical Research

Affiliated Institutions
  • Menzies Institute for Medical Research, Hobart, Australia

Source

Donor Derived

Age: 65-69

Biological Sex: Male

Disease: EFO:0008520
Label: primary progressive multiple sclerosis
Definition: A multiple sclerosis that is characterized by steady worsening of neurologic functioning, without any distinct relapses or periods of remission. The rate of progression may vary over time, with occasional plateaus or temporary improvements, but the progression is continuous.| Primary progressive multiple sclerosis is the form of the multiple sclerosis in which clinical disability progression occurs continuously without remissions.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2021

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: No

Ethics

Ethics Number: H0016915

Institution Human Research Ethics Council: University of Tasmania Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 4

Karyotype: 46,XY

Karyotype Method: G-banding

Summary: 20 metaphase spreads were analysed.

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
HIV 1 Negative
HIV 2 Negative
HEP B Negative
HEP C Negative
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
NANOG RT-PCR
TRA-1-81 Flow cytometry
POU5F1 (OCT-4) RT-PCR
SOX2 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: AFP

Mesoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: HAND1

Ectoderm

In vitro spontaneous differentiation

Assessed by: Immunostaining

Markers: PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 20%

Medium Items:

  • Base Medium: mTeSR Plus - STEMCELL Technologies
  • Base Coat: Matrigel

Passage Method: Enzymatically

External References

Source
Stem cell research & therapy Induced pluripotent stem cell derived pericytes respond to mediators of proliferation and contractility
Frontiers in Immunology 10.3389/fimmu.2024.1349138
Stem cell research Generation and characterisation of four multiple sclerosis iPSC lines from a single family
Stem cell research Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis
Stem Cell Research Generation of MNZTASi001-A, a human pluripotent stem cell line from a person with primary progressive multiple sclerosis.
hPSCreg MNZTASi001-A
Cellosaurus CVCL_B5IS
BioSamples SAMEA112762629
Wikidata Q110433060
Phenomics Australia Phenomics Australia