UOWi001-A

Not yet reviewed by Australian Stem Cell Registry

iPSC RB 9–8, RB 9-8, RB9-8, HC3

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma7163
Label: Skin
Definition: Nonparenchymatous organ that consists of the epidermis, dermis and sets of hair and nails and which surrounds the entire body. Subdivisions of the skin surround various body parts; as a whole, the skin constitutes the external layer of the body. Examples: There is only one skin.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Line Custodianship

Cell Line Maintainer

Ooi

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia
View all cell lines from this group

Cell Line Producer

University of Wollongong

Affiliated Institutions
  • University of Wollongong, Wollongong City Council, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: UBERON:0002067
Label: dermis
Definition: The dermis is a layer of skin between the epidermis (with which it makes up the skin) and subcutaneous tissues, and is composed of two layers, the papillary and reticular dermis[WP].

Derivation Year: 2017

Ethics

Ethics Number: HE13/299

Institution Human Research Ethics Council: University of Wollongong Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XX

Karyotype Method: G-banding

Summary: Karyotyping occurred at a resolution of 400 bphs.

STR analysis Results:

Loci Allele 1 Allele 2
D5S818 11 12
D13S317 11 None
D7S820 10 11
D16S539 11 12
VWA 16 17
TH01 6 7
Amelogenin X None
TPOX 8 9
CSF1PO 11 12
D8S1179 13 14
D21S11 30.2 31
D3S1358 15 None
D2S1338 19 23
D19S433 12 13
D18S51 13 None
FGA 23 24
PENTA E 12 13
PENTA D 10 OL

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
POU5F1 (OCT-4) Immunostaining
SSEA-4 Immunostaining
TRA-1-60 Immunostaining
NANOG RT-PCR
POU5F1 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

Classification Result
Self-renewal False
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency Characterisation

Endoderm

Unknown

Assessed by: Unavailable

Markers: None

Mesoderm

Unknown

Assessed by: Unavailable

Markers: None

Ectoderm

Unknown

Assessed by: Unavailable

Markers: None

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: 5%

Medium Items:

  • Base Coat: Matrigel - Corning
  • Base Medium: TeSR-E8 - STEMCELL Technologies

Passage Method: Enzymatically

External References