1502, 1502.3 , MCRIAi001-A
No genetic information available for this cell line
Lamandé
Murdoch Children's Research Institute
Source Cell Type:
CL:0000057
Label: fibroblast
Definition:
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
Source Cell Origin:
UBERON:0002067
Label: dermis
Definition:
The dermis is a layer of skin between the epidermis (with which it makes up the skin) and subcutaneous tissues, and is composed of two layers, the papillary and reticular dermis[WP].
Derivation Year: 2019
Ethics Number: 33118
Institution Human Research Ethics Council: Royal Children's Hospital Human Research Ethics Committee
Approval Date: To be verified
No genomic modifications available for this line.
Passage Number: 10
Karyotype: arr(1-22,X)x2
Karyotype Method: Molecular karyotyping by SNP array
Summary: Molecular karyotyping by SNP array (Illumina Infinium GSA-24; resolution 0.5 Mb).
| Disease | Result |
|---|---|
| Mycoplasma | Negative |
| Marker | Method |
|---|---|
| NANOG | Immunostaining |
| POU5F1 (OCT-4) | Immunostaining |
| OCT3/4 | Flow cytometry |
| SSEA-4 | Flow cytometry |
| TRA-1-60 | Flow cytometry |
No available data for this cell line.
No available data for this cell line.
In vitro directed differentiation
Assessed by: Immunostaining
Markers: SOX17
In vitro directed differentiation
Assessed by: RT-PCR
Markers: COL2A1;FOXC2;MEOX1;SOX9
In vitro directed differentiation
Assessed by: Immunostaining
Markers: NES;PAX6
CO2 concentration: 5%
O2 concentration: Unavailable
Medium Items:
Passage Method: Enzyme-free cell dissociation