POWHe002-A

Reviewed by Australian Stem Cell Registry

NIHhESC-10-0073, Endeavour 2, E-2

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma83041
Label: Blastocyst
Definition: Developmental organism which has as its parts trophoblast, embryoblast and blastocystic cavity.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
In vitro cellular & developmental biology. Animal 2010 Derivation of a new human embryonic stem cell line, Endeavour-2, and its characterization
In Vitro Cellular & Developmental Biology - Animal 2010 Derivation of a new human embryonic stem cell line, Endeavour-2, and its characterization.

Line Custodianship

Cell Line Maintainer

Kuldip Sidhu

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Cell Line Producer

Prince of Wales Hospital

Affiliated Institutions
  • Prince of Wales Hospital, Sydney, Australia

Source

Donor Derived

Age: Embryo

Biological Sex: Female

Derivation Details

Embryonic Derivation Details

NHMRC Licence Number: 309708.0

Genetic Diagnosis: False

Supernumerary from IVF: False

Derivation Year: None

Ethics

Ethics Number: HREC 02247/02267

Institution Human Research Ethics Council: Holding Entry

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 9

Karyotype: 46,XX

Karyotype Method: G-banding

Summary: 20 metaphase spreads were analysed.

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

Marker Method
NANOG RT-PCR
POU5F1 (OCT-4) RT-PCR
ITGA6 Flow cytometry
SSEA-4 Flow cytometry
TRA-1-60 Flow cytometry
Alkaline Phosphatase Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vivo teratoma

Assessed by: nan

Markers: nan

Mesoderm

In vivo teratoma

Assessed by: nan

Markers: nan

Ectoderm

In vivo teratoma

Assessed by: nan

Markers: nan

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Gelatin - Invitrogen
  • Base Coat: Gamma-irradiated human fetal fibroblasts

Passage Method: Enzymatically