MICCNi001-B

Not yet reviewed by Australian Stem Cell Registry

hiPSC_03 family control sibling, clone C2, ADHD Stem Cell Study_subject 6

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Stem cell research 2019 Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family

Line Custodianship

Cell Line Maintainer

Tong

View all cell lines from this group

Cell Line Producer

Monash Institute of Cognitive and Clinical Neurosciences

Affiliated Institutions
  • Monash Institute of Cognitive and Clinical Neurosciences, Clayton, Australia

Source

Donor Derived

Age: 15-19

Biological Sex: Male

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2018

Reprogramming Method

Vector Type: Not integrated

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: No

Ethics

Ethics Number: CF15/2566 - 2015001048

Institution Human Research Ethics Council: Monash University Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 10

Karyotype: 46,XY

Karyotype Method: G-banding

Summary: 15 cells were analysed (metaphase spread: 5; chromosome analysis: 10).

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
TRA 1-60 Immunostaining
NANOG Immunostaining
Klf4 Immunostaining
POU5F1 (OCT-4) Immunostaining
OCT3/4 Immunostaining
TRA-1-60 Immunostaining
POU5F1 RT-PCR
SOX2 RT-PCR

Scorecard Results

Undifferentiated Cells

Classification Result
Self-renewal False
Endoderm False
Mesoderm False
Ectoderm True

Pluripotency

Classification Result
Self-renewal False
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency Characterisation

Endoderm

None

Assessed by: Unavailable

Markers:

Mesoderm

Unknown

Assessed by: Unavailable

Markers: None

Ectoderm

Unknown

Assessed by: Unavailable

Markers: None

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Medium: Essential 8 Medium - Invitrogen
  • Base Coat: Vitronectin - Thermo Fisher Scientific Inc.
  • Supplement: Rock inhibitor (at passage) - Merck-Millipore

Passage Method: Enzyme-free cell dissociation