MCRIi019-A-1

Extracted from hPSCreg

1502.3 miR-26b KO, 1502-miR26b knockout

Details

Tissue & Disease as reported

Disease as reported (Genomic Modifications)

Genetic Information

Genotype Locus
MIR26B
Polymorphism
Homozygous deletion of miR-26b stem-loop
Modifications
Gene Knock-out

Associated Publications

Journal Year Article
Stem Cell Research 2021 Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing.
Stem cell research 2020 Generation of a miR-26b stem-loop knockout human iPSC line, MCRIi019-A-1, using CRISPR/Cas9 editing

Line Custodianship

Cell Line Maintainer

Bateman-MCRI

View all cell lines from this group

Cell Line Producer

Murdoch Children's Research Institute

Affiliated Institutions
  • Murdoch Children's Research Institute, Melbourne, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: N/A

Source Cell Origin: N/A

Derivation Year:

Ethics

Ethics Number: 33118

Institution Human Research Ethics Council: Royal Children's Hospital Human Research Ethics Committee

Approval Date: To be verified

Modifications

Genomic Modifications

Homozygous deletion of miR-26b stem-loop

Homozygous deletion of MIR26B stem-loop region removing the sequences containing miR-26b-5p and miR-26b-3p in both alleles (allele 1: 121 bp deletion; allele 2: 132 bp deletion).

Cytoband: 2q.35
Mutation Type: Gene Knock-out
Delivery Method: CRISPR/Cas9

Quality Assurance

Genomic Characterisation

Passage Number: 21

Karyotype: arr(1-22,X)x2

Karyotype Method: Molecular karyotyping by SNP array

Summary: Molecular karyotyping by SNP array (Illumina Infinium GSA-24 v1.0; resolution 0.5 Mb). SNPduo comparison to parental cell (>99.9% identity).

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
OCT4 Immunostaining
OCT3/4 Flow cytometry
SSEA-4 Flow cytometry
TRA-1-60 Flow cytometry

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: SOX17

Mesoderm

In vitro directed differentiation

Assessed by: RT-qPCR

Markers: COL2A1; FOXC2; MEOX1; SOX9

Ectoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: NES; PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Matrigel - Corning
  • Base Medium: Essential 8 Medium - Thermo Fisher Scientific Inc.

Passage Method: Enzyme-free cell dissociation