LEIi004-A

Not yet reviewed by Australian Stem Cell Registry

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma7163
Label: Skin
Definition: Nonparenchymatous organ that consists of the epidermis, dermis and sets of hair and nails and which surrounds the entire body. Subdivisions of the skin surround various body parts; as a whole, the skin constitutes the external layer of the body. Examples: There is only one skin.
Tissue for derived iPSC
Disease as reported (Donor)
retinitis pigmentosa

Genetic Information

Genotype Locus
CLN3
Polymorphism
Compound heterozygous: NM_000086.2:c.175G>A(p.Ala59Thr); and: unspecified_reference_CLN3: 1.02kb deletion including of exons 7 and 8

Associated Publications

Journal Year Article
Molecules (Basel, Switzerland) 2021 Patient-Derived Induced Pluripotent Stem Cell Models for Phenotypic Screening in the Neuronal Ceroid Lipofuscinoses
Molecular genetics & genomic medicine 2021 Gene correction of the CLN3 c.175G>A variant in patient-derived induced pluripotent stem cells prevents pathological changes in retinal organoids
Molecular Genetics and Genomic Medicine 2021 Gene correction of the CLN3 c.175G>A variant in patient-derived induced pluripotent stem cells prevents pathological changes in retinal organoids.
Stem cell research 2018 Generation of an induced pluripotent stem cell line from a patient with non-syndromic CLN3-associated retinal degeneration and a coisogenic control line
Stem Cell Research 2018 Generation of an induced pluripotent stem cell line from a patient with non-syndromic CLN3-associated retinal degeneration and a coisogenic control line.

Line Custodianship

Cell Line Maintainer

Chen

Affiliated Institutions
  • Lions Eye Institute, Nedlands, Australia
View all cell lines from this group

Cell Line Producer

Lions Eye Institute

Affiliated Institutions
  • Lions Eye Institute, Nedlands, Australia

Source

Donor Derived

Age: 50-54

Biological Sex: Female

Disease: MONDO:0019200
Label: retinitis pigmentosa
Definition: Retinitis pigmentosa (RP) is an inherited retinal dystrophy leading to progressive loss of the photoreceptors and retinal pigment epithelium and resulting in blindness usually after several decades.

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000057
Label: fibroblast
Definition: A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.

Source Cell Origin: UBERON:0002067
Label: dermis
Definition: The dermis is a layer of skin between the epidermis (with which it makes up the skin) and subcutaneous tissues, and is composed of two layers, the papillary and reticular dermis[WP].

Derivation Year: 2016

Reprogramming Method

Vector Type: Not integrated

Vector: Episomal

Kit: [kit] Episomal iPSC Reprogramming Plasmids

Detected: Unknown

Ethics

Ethics Number: 2001–053

Institution Human Research Ethics Council: Sir Charles Gairdner Hospital Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 25

Karyotype: arr(1-22,X)x2

Karyotype Method: Molecular karyotyping by SNP array

Summary: Molecular karyotyping by SNP array (Illumina HumanCoreExome-24 Beadchip; resolution 0.5 Mb).

STR analysis Results:

Loci Allele 1 Allele 2
CSF1PO None None
D13S317 None None
D16S539 None None
D18S51 None None
D21S11 None None
D3S1358 None None
D5S818 None None
D7S820 None None
D8S1179 None None
FGA None None
PENTA D None None
PENTA E None None
TH01 None None
TPOX None None
VWA None None
Amelogenin None None

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
NANOG Immunostaining
POU5F1 (OCT-4) Immunostaining
SOX2 Immunostaining
SSEA-4 Immunostaining
KLF4 Immunostaining
TRA-1-81 Immunostaining
C-MYC RT-PCR
KLF4 RT-PCR
NANOG RT-PCR
POU5F1 (OCT-4) RT-PCR
SOX2 RT-PCR

Scorecard Results

Undifferentiated Cells

Classification Result
Self-renewal True
Endoderm True
Mesoderm True
Ectoderm True

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro spontaneous differentiation

Assessed by: RT-PCR

Markers: Brachyury;FDGFRB

Mesoderm

In vitro spontaneous differentiation

Assessed by: RT-PCR

Markers: FOXA2;GATA4;SOX17

Ectoderm

In vitro spontaneous differentiation

Assessed by: RT-PCR

Markers: OTX1;PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: 20%

Medium Items:

  • Base Medium: TeSR-E8 - STEMCELL Technologies
  • Base Coat: Geltrex - ThermoFisher

Passage Method: Enzyme-free cell dissociation

External References

Source
Molecules (Basel, Switzerland) Patient-Derived Induced Pluripotent Stem Cell Models for Phenotypic Screening in the Neuronal Ceroid Lipofuscinoses
Molecular genetics & genomic medicine Gene correction of the CLN3 c.175G>A variant in patient-derived induced pluripotent stem cells prevents pathological changes in retinal organoids
Molecular Genetics and Genomic Medicine Gene correction of the CLN3 c.175G>A variant in patient-derived induced pluripotent stem cells prevents pathological changes in retinal organoids.
Stem cell research Generation of an induced pluripotent stem cell line from a patient with non-syndromic CLN3-associated retinal degeneration and a coisogenic control line
Stem Cell Research Generation of an induced pluripotent stem cell line from a patient with non-syndromic CLN3-associated retinal degeneration and a coisogenic control line.
hPSCreg LEIi004-A
Cellosaurus CVCL_VE61
BioSamples SAMEA104626352
Wikidata Q54902437
Phenomics Australia Phenomics Australia