WAe009-A-24

Extracted from hPSCreg

Details

Tissue & Disease as reported

Disease as reported (Genomic Modifications)

Genetic Information

Modifications
Gene Knock-in

Associated Publications

Journal Year Article
Stem cell reports 2020 A CX3CR1 Reporter hESC Line Facilitates Integrative Analysis of In-Vitro-Derived Microglia and Improved Microglia Identity upon Neuron-Glia Co-culture

Line Custodianship

Cell Line Maintainer

Monash Institute of Pharmaceutical Sciences

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Cell Line Producer

Monash Institute of Pharmaceutical Sciences

Affiliated Institutions
  • Monash Institute of Pharmaceutical Sciences, Melbourne, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: N/A

Source Cell Origin: N/A

Derivation Year:

Ethics

Ethics Number: Holding Entry-WAe009-A

Institution Human Research Ethics Council: Holding Entry

Approval Date: None

Modifications

Genomic Modifications

This line is engineered to include a genetic reporter of CX3CR1 expression which results in expression of tdTomato and nanoluciferase in series with CX3CR1 expression. The construct also includes a neomycin resistance cassette flanked by FRT sites. One allele of the CX3CR1 gene contains the following construct targeted to the stop codon: IRES-tdTomato-T2A-Nanoluc-pA-FRT-neo-FRT.

Cytoband: 3p22.2
Mutation Type: Gene Knock-in

Quality Assurance

Genomic Characterisation

Passage Number:

Karyotype:

Karyotype Method:

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

No available data for this cell line.

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

Passage Method: Not specified