CIAUi003-A-1

Extracted from hPSCreg

BFC4-C, BFC4-CRISPR-corrected

Details

Tissue & Disease as reported

Disease as reported (Genomic Modifications)
catecholaminergic polymorphic ventricular tachycardia 2 (Variant) catecholaminergic polymorphic ventricular tachycardia 2 (Variant)

Genetic Information

Modifications
Variant Variant

Associated Publications

No available publications

Line Custodianship

Cell Line Maintainer

Centenary Institute of Cancer Medicine and Cell Biology

View all cell lines from this group

Cell Line Producer

Centenary Institute of Cancer Medicine and Cell Biology

Affiliated Institutions
  • Centenary Institute of Cancer Medicine and Cell Biology, Camperdown, Australia

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:0000842
Label: mononuclear leukocyte
Definition: A leukocyte with a single non-segmented nucleus in the mature form.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year:

Reprogramming Method

Vector Type: Not integrated

Vector: Episomal

Ethics

Ethics Number: X19-0108 and 2019/ETH00461

Institution Human Research Ethics Council: Ethics Review Committee, Sydney Local Health District

Approval Date: To be verified

Modifications

Genomic Modifications

Any catecholaminergic polymorphic ventricular tachycardia in which the cause of the disease is a mutation in the CASQ2 gene.

Cytoband: chr1(GRCh38):g.115732968T>C
Mutation Type: Variant

Any catecholaminergic polymorphic ventricular tachycardia in which the cause of the disease is a mutation in the CASQ2 gene.

Cytoband: chr1(GRCh38):g.115732968T>C
Mutation Type: Variant

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: Normal

Karyotype Method: Molecular karyotyping by SNP array

STR analysis Results:

Performed but not available.

Microbiology and Virology Screening

Disease Result
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
OCT4 Immunostaining
SSEA-4 Immunostaining
TRA 1-60 Immunostaining
SOX2 Immunostaining

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Unavailable

Markers: CXCR4;SOX17

Mesoderm

In vitro directed differentiation

Assessed by: Unavailable

Markers: TNNT2;NKX2.5

Ectoderm

In vitro directed differentiation

Assessed by: Unavailable

Markers: NESTIN;PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: 5%

O2 concentration: Unavailable

Medium Items:

Passage Method: Not specified