GENEAe002-A

Extracted from hPSCreg

GENEA003, SIVF03, SIVF003

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma83041
Label: Blastocyst
Definition: Developmental organism which has as its parts trophoblast, embryoblast and blastocystic cavity.
Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal Year Article
Cloning and stem cells 2008 Karyotypically normal and abnormal human embryonic stem cell lines derived from PGD-analyzed embryos
Cloning and Stem Cells 2008 Karyotypically normal and abnormal human embryonic stem cell lines derived from PGD-analyzed embryos.

Line Custodianship

Cell Line Maintainer

Schmidt-GENEA

View all cell lines from this group

Cell Line Producer

Genea

Affiliated Institutions
  • Genea, San Diego, United States

Source

Donor Derived

Age: Embryo

Biological Sex: Female

Derivation Details

Embryonic Derivation Details

NHMRC Licence Number:

Genetic Dignosis:

Supernumerary from IVF:

Derivation Year:

NHMRC Licence Number:

Genetic Dignosis:

Supernumerary from IVF:

Derivation Year:

Ethics

Ethics Number: Holding Entry-GENEAe002-A

Institution Human Research Ethics Council: Sydney IVF Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 55

Karyotype: 47,XX,+16

Karyotype Method: G-banding

Summary: Karyotyping occurred at passages 6, 29 and 55.

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

Marker Method
Alkaline Phosphatase Flow cytometry
POU5F1 (OCT-4) Flow cytometry
SSEA-1 Flow cytometry
SSEA-4 Flow cytometry
TRA 1-60 Flow cytometry
Alkaline phosphatase Immunostaining
OCT4 Immunostaining
SSEA-4 Immunostaining
TRA-1-60 Immunostaining
OCT4 RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vivo teratoma

Assessed by: Unavailable

Markers:

In vitro spontaneous differentiation

Assessed by: RT-PCR

Markers: AFP

In vivo teratoma

Assessed by: nan

Markers: nan

Mesoderm

In vivo teratoma

Assessed by: Unavailable

Markers:

In vitro spontaneous differentiation

Assessed by: RT-PCR

Markers: VEGFR2

In vivo teratoma

Assessed by: nan

Markers: nan

Ectoderm

In vitro spontaneous differentiation

Assessed by: Unavailable

Markers:

In vitro spontaneous differentiation

Assessed by: RT-PCR

Markers: NCAM

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

  • Base Medium: KnockOut DMEM (Dulbecco's Modified Eagle Medium) - Gibco BRL
  • Supplement: Glutamine - Gibco BRL
  • Supplement: Penicillin - Gibco BRL
  • Supplement: Streptomycin - Gibco BRL
  • Supplement: MEM Amino Acid Solution - Gibco BRL
  • Supplement: 2-mercaptoethanol - Gibco BRL
  • Serum: Knock-out Serum Replacement - Gibco BRL
  • Main Protein: basic fibroblast growth factor - Gibco BRL

Passage Method: Mechanically