VCCRIi001-A

Extracted from hPSCreg

Details

Tissue & Disease as reported

Biopsy Location
Ontology ID: fma9670
Label: Portion of blood
Definition: Portion of body substance which has as its parts plasma and blood cells.
Tissue for derived iPSC
Disease as reported (Donor)
Spontaneous coronary artery dissection

Genetic Information

Genotype Locus
PHACTR1
Polymorphism
Heterozygous: NC_000006.12:g.12903725A>G

Associated Publications

Journal Year Article
Medicina 2024 10.3390/medicina60020217
Stem cell research 2023 Generation of induced pluripotent stem cell lines from a sister pair who suffered post-partum or recurrent Spontaneous Coronary Artery Dissections
Heart, lung & circulation 2021 Spontaneous Coronary Artery Dissection and Fibromuscular Dysplasia: Vasculopathies With a Predilection for Women
Stem cell research 2019 Generation of VCCRIi001-A, a human induced pluripotent stem cell line, from a patient with spontaneous coronary artery dissection
Stem Cell Research 2019 Generation of VCCRIi001-A, a human induced pluripotent stem cell line, from a patient with spontaneous coronary artery dissection.

Line Custodianship

Cell Line Maintainer

Graham-VCCRI

View all cell lines from this group

Cell Line Producer

Victor Chang Cardiac Research Institute

Affiliated Institutions
  • Victor Chang Cardiac Research Institute, Sydney, Australia

Source

Donor Derived

Age: 45-49

Biological Sex: Female

Disease: MONDO:0007385
Label: idiopathic spontaneous coronary artery dissection
Definition:

Derivation Details

Induced Pluripotent Cell Derivation Details

Source Cell Type: CL:2000001
Label: peripheral blood mononuclear cell
Definition: A leukocyte with a single non-segmented nucleus in the mature form found in the circulatory pool of blood.

Source Cell Origin: UBERON:0000178
Label: blood
Definition: A fluid that is composed of blood plasma and erythrocytes.

Derivation Year: 2017

Reprogramming Method

Vector Type:

Vector: Sendai Virus

Kit: [kit] CytoTune-iPS 2.0 Sendai reprogramming kit

Detected: {{ details.non_integrated_vector.get_detected_reprogramming_vector_display }}

Ethics

Ethics Number: HREC/16/SVH/338; SVH File number: 16/245

Institution Human Research Ethics Council: St Vincent's Hospital Human Research Ethics Committee

Approval Date: To be verified

Modifications

Genomic Modifications

Genotype: PHACTR1
Polymorphism: Heterozygous: NC_000006.12:g.12903725A>G

Quality Assurance

Genomic Characterisation

Passage Number: Not available

Karyotype: 46,XX

Karyotype Method: G-banding

STR analysis Results:

Loci Allele 1 Allele 2
D5S818 12 14
D13S317 11 13
D7S820 10 8
D16S539 11 None
VWA 17 18
TH01 9 9.3
Amelogenin X None
TPOX 8 None
CSF1PO 12 9
D8S1179 10 16
D21S11 29 32.2
D3S1358 15 18
D2S1338 17 None
D19S433 15 None
D18S51 12 16
FGA 21 24
PENTA E 11 7
PENTA D 12 9

Microbiology and Virology Screening

Disease Result
HIV 1 Negative
HIV 2 Negative
HEP B Negative
HEP C Negative
Mycoplasma Negative

Characterisation of Undifferentiated Cells

Marker Method
OCT4 Immunostaining
NANOG RT-qPCR
POU5F1 RT-qPCR
SOX2 RT-qPCR
TRA-1-60 Flow cytometry

Scorecard Results

Undifferentiated Cells

No available data for this cell line.

Pluripotency

No available data for this cell line.

Pluripotency Characterisation

Endoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: SOX17

Mesoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: GATA4

Ectoderm

In vitro directed differentiation

Assessed by: Immunostaining

Markers: PAX6

Growth Characteristics

Culture Medium and Growth Conditions

CO2 concentration: Unavailable

O2 concentration: Unavailable

Medium Items:

  • Base Coat: Matrigel - Corning
  • Base Medium: mTeSR1 Plus - STEMCELL Technologies

Passage Method: Not specified

External References

Source
Medicina 10.3390/medicina60020217
Stem cell research Generation of induced pluripotent stem cell lines from a sister pair who suffered post-partum or recurrent Spontaneous Coronary Artery Dissections
Heart, lung & circulation Spontaneous Coronary Artery Dissection and Fibromuscular Dysplasia: Vasculopathies With a Predilection for Women
Stem cell research Generation of VCCRIi001-A, a human induced pluripotent stem cell line, from a patient with spontaneous coronary artery dissection
Stem Cell Research Generation of VCCRIi001-A, a human induced pluripotent stem cell line, from a patient with spontaneous coronary artery dissection.
hPSCreg VCCRIi001-A
Cellosaurus CVCL_YC64
Wikidata Q98134501
Phenomics Australia Phenomics Australia