Australian Human Pluripotent Stem Cell Registry

GENEAe004-A

Extracted from hPSCreg

GENEA005, SIVF05, SIVF005

Details

Tissue & Disease as reported

Biopsy Location

Ontology ID: fma83041

Label: Blastocyst

Definition: Developmental organism which has as its parts trophoblast, embryoblast and blastocystic cavity.

Tissue for derived iPSC

Genetic Information

No genetic information available for this cell line

Associated Publications

Journal	Year	Article
Cloning and stem cells	2008	Karyotypically normal and abnormal human embryonic stem cell lines derived from PGD-analyzed embryos
Cloning and Stem Cells	2008	Karyotypically normal and abnormal human embryonic stem cell lines derived from PGD-analyzed embryos.

Line Custodianship

Cell Line Maintainer

Schmidt-GENEA

View all cell lines from this group

Cell Line Producer

Genea

Affiliated Institutions

Genea, San Diego, United States

Source

Donor Derived

Age: Embryo

Biological Sex: Female

Derivation Details

Embryonic Derivation Details

NHMRC Licence Number:

Genetic Dignosis:

Supernumerary from IVF:

Derivation Year:

NHMRC Licence Number:

Genetic Dignosis:

Supernumerary from IVF:

Derivation Year:

Ethics

Ethics Number: Holding Entry-GENEAe004-A

Institution Human Research Ethics Council: Sydney IVF Human Research Ethics Committee

Approval Date: To be verified

Modifications

No genomic modifications available for this line.

Quality Assurance

Genomic Characterisation

Passage Number: 28

Karyotype: 46XX

Karyotype Method: G-banding

Summary: Karyotyping occurred at passages 8 and 28.

Microbiology and Virology Screening

No available data for this cell line.

Characterisation of Undifferentiated Cells

Marker	Method
Alkaline Phosphatase	Flow cytometry
POU5F1 (OCT-4)	Flow cytometry
SSEA-4	Flow cytometry
TRA 1-60	Flow cytometry
Alkaline phosphatase	Immunostaining
OCT4	Immunostaining
SSEA-4	Immunostaining
TRA-1-60	Immunostaining
OCT4	RT-PCR

Scorecard Results

Undifferentiated Cells

No available data for this cell line.